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ceacam 1 his  (Sino Biological)


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    Structured Review

    Sino Biological ceacam 1 his
    The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), <t>CEACAM-1/TIM-3</t> (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.
    Ceacam 1 His, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ceacam 1 his/product/Sino Biological
    Average 93 stars, based on 4 article reviews
    ceacam 1 his - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands"

    Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28126

    The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), CEACAM-1/TIM-3 (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.
    Figure Legend Snippet: The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), CEACAM-1/TIM-3 (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control



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    ceacam 1 his  (Sino Biological)
    93
    Sino Biological ceacam 1 his
    The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), <t>CEACAM-1/TIM-3</t> (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.
    Ceacam 1 His, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ceacam 1 his/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    ceacam 1 his - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

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    The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), CEACAM-1/TIM-3 (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.

    Journal: Heliyon

    Article Title: Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands

    doi: 10.1016/j.heliyon.2024.e28126

    Figure Lengend Snippet: The binding characteristics of anti-TIM-3 antibodies to ligand/TIM-3 complex. The binding of mouse anti-human TIM-3 antibodies to human Gal-9/TIM-3 (B), CEACAM-1/TIM-3 (C) or HMGB-1/TIM-3 complex (D) was detected using anti-mouse antibodies in ELISAs. Prior to being treated with mouse anti-TIM-3 mAbs, TIM-3-His were firstly attached to immobilized Gal-9, CEACAM-1, or HMGB-1 in ELISA plates. Reaction complexes were identified using anti-Ms-(H + L)-HRP. NC = negative control without TIM-3 protein; Blank = blank control without an anti-TIM-3 antibody. Horizontal lines indicate the cut-off, which were determined based on 2.1 times the mean absorbance of the negative control or blank. Statistical significance was indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 compared with NC or Blank.

    Article Snippet: Recombinant proteins, including CEACAM-1-His (Sino Biological, 10822-H08H) and HMGB-1-His (Sino Biological, 10326-H08H), were immobilized on ELISA plates at a concentration of 1 μg/mL and incubated overnight at 4 °C.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control